WHO Laboratory Manual for the Examination and Processing of Human Semen - July 2010 Edition



Semen analysis may be useful in both clinical and research settings, for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility
regulation and other interventions. This manual provides updated,
standardized, evidence-based procedures and recommendations for
laboratory managers, scientists and technicians to follow in examining
human semen in a clinical or research setting. Detailed protocols for
routine, optional and research tests are elaborated.
This new edition comprises three parts: semen analysis, sperm
preparation and quality assurance. The text includes descriptions of how
to construct a conventional semen profile and provides standardized
protocols for performing several optional diagnostic procedures. Such
techniques are essential in the evaluation of infertile couples and in
assessing fertility in men whose sperm production is suppressed by
potential anti-fertility compounds or by toxic agents: they are also of
interest in forensic medicine and in connection with artificial
insemination.
The fifth edition includes new information on sperm preparation for
clinical use or specialized assays and on cyropreservation, an expanded section
on quality control in the semen analysis laboratory and evidence-based
reference ranges and reference limits for various semen characteristics.
The methods described are intended to improve the quality of semen
analysis and the comparability of results from different laboratories.
Previous editions of this volume have been recognized as providing
global standards in the area of fertility investigation and treatment,
and have been used extensively by research and clinical laboratories
throughout the world

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