The ELISA Guidebook

The Enzyme Linked Immunosorbent Assay
(ELISA) continues, as predicted, to be the predominant assay used in
biomedical and agricultural research. While little has changed in the
principles of the ELISAs used since the publication of The ELISA
Guidebook, many technological advantages, including the increasing
number of reagents available for exploitation and the larger spectrum of
instruments to measure the coloured products of ELISAs, have greatly
benefited the field. Other testing technologies using molecular
techniques, predominantly the conventional and more and more often, real
time PCR, are rapidly evolving to help diagnose and differentiate
disease agents and identify substances present in exquisitely small
amounts. The ELISA is still needed either alone in mass screening, such
as serological assays, or used in tandem with molecular techniques to
fully evaluate the molecular and antigenic nature of analytes.
John
R. Crowther provides today’s premier practical guide to the
understanding and application of ELISA. This Edition enlarges on
charting methods for assessing the indirect ELISA, ruggedness and
robustness of tests, aspects of kit use and validation, and internal
quality control and external quality management of data. The author
describes each method in great detail to ensure experimental success and
includes advice on equipment choice, maintenance, and calibration.  
With its numerous worked examples, detailed instructions,
and extensive illustrations, The ELISA Guidebook, Second Edition offers a
powerful synthesis of all the basic concepts and practical experimental
details investigators need to understand, develop, and apply ELISA
methodology successfully in day-to-day basic and clinical research

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